Summary
Type: SPE
Application #: 304920
Catalog#: 730952 - Chromab. Columns HR-XC, 3 ml, 200 mg
Detection:
Conditions: Column conditioning: 5 mL methanol, then 5 mL water
(Do not let run the column dry.)
Sample aspiration: The prepared sample is passed through the column by gravity or low vacuum
Washing: 5 mL 0.1 mol/L HCl, then 5 mL methanol
Drying: 10 min vacuum
Elution: 2 x 2.5 mL methanol / 5 % NH3
Evaporation with nitrogen stream and reconstitution with a suitable solvent for the subsequent analysis
Authors: SPE department
Source: Macherey-Nagel (Germany 2008)